Instabilities and geometry of growing tissues

We present a covariant continuum formulation of a generalized two-dimensional vertex like model of epithelial tissues which describes tissues with different underlying geometries, and allows for an analytical macroscopic description. Using a geometrical approach and out-of-equilibrium statistical mechanics, we calculate both mechanical and dynamical instabilities of a tissue, and their dependences on various variables, including activity, and cell-shape heterogeneity (disorder). We show how both plastic cellular rearrangements and the tissue elastic response depend on the existence of mechanical residual stresses at the cellular level. Even freely growing tissues may exhibit a growth instability depending on the intrinsic proliferation rate. Our main result is an explicit calculation of the cell pressure in a homeostatic state of a confined growing tissue. We show that the homeostatic pressure can be negative and depends on the existence of mechanical residual stresses. This geometric model allows us to sort out elastic and plastic effects in a growing, flowing, tissue.